Central Ohio Technical College Biology Lab Report

McKenna – Spokesperson
Autumn – Recorder
Tiffany – Facilitator
Table test 1: Benedictïstarch – testing for monosaccharides (benedicté and polysaccharides
(starch) (positive result = blue/black and negative result = yellow for starch) (some sugar =
green/yellow and large amount of sugar = orange/red)
Tube 1
Tube 2
Tube 3
Tube 4
Tube 5
Tube 6
Tube 7
H2o &
starch
H2o &
Amylase
H2o &
maltose
Starch &
amylase
Starch
&boiled
amylase
Starch
&amylas
e
Starch &
amylase
37 C
37 C
37 C
37 C
37 C
37 C
0C
Sugar
results
Negative
– blue
Positive dark
orange
Positive red/orang
e
Negative
– blue
Positive dark
ornage
Positive dark
orange
Positive dark
orange
Starch
results
Positive – Negative
Dark blue – yellow
Negative
– dark
orange
Positive dark blue
Postitive
– dark
purple
Weak
positive brown/
orange
Positive dark
purple
Tube 2 – should be negative, possible contamination
Tube 4 – should¥ been positive, did not work
Table test 2: Biuret – testing for amino acids (positive test = pink/purple and negative test = blue)
Color of
solution
after
adding
biuret
Tube 1
Tube 2
Tube 3
Tube 4
Tube 5
Tube 6
H2O &
albumin
H2O &
pepsin
H2O &
albumin,
pepsin
Albumin & Albumin
tripson
and boiled
pepsin
Albumin
and pepsin
37 C
37 C
37 C
37 C
37 C
0C
purple
clear/blue
Light
purple
Dark
purple
Dark
purple
Dark
purple
McKenna – Spokesperson
Autumn – Recorder
Tiffany – Facilitator
Protein
digestion
(yes or no)
Positive
Negative
Positive
Positive
Positive
Positive
For Biruet test – add 2mL 10% NaOH then 5 drops CuSO4
Table test 3: chemical testing – testing for fatty acids ( acid present = pink, acid absent = purple)
Tube 1
Tube 2
Tube 3
Tube 4
Tube 5
Tube 6
H2O &
lipase
H2O &
litmus
Litmus &
lipase
Litmus &
lipase &
bile
Litmus &
boiled
lipase
Litmus &
lipase
37 C
37 C
37 C
37 C
37 C
0C
Color of
contents
after
incubation
Clear
purple
purple
pink
pink
Pink
Fat
digestion
(yes or no)
Negative
Negative
Negative
Positive
Positive
Positive
McKenna – Spokesperson
Autumn – Recorder
Tiffany – Facilitator
Part 1
Enzymes only work at 37 degrees C.—– Hypothesis
Gluscose we would reject the hypothesis.
Lipase we would reject the hypothesis.
Part 2
Was the experiment set up correctly?
What we need: 3x tables (carbs, proteins, lipids)
results : talk about controls, Did they work properly?
Discussion/ Conclusion: Did we support the hypothesis? Give evidence, talk about tubes and
outcomes.
Any unusual results( dont just say contamination explain what could of happen to cause unusual
results)
Answer why are digestive enzymes important.
Our team after conducting the experiment given would not support the hypothesis

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